CST, 4217T, USP28 Antibody

Polyclonal Antibody for studying USP28. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, USP28 Antibody (CST #4217) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity USP28 Antibody detects transfected levels of USP28 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala11 of human USP28. Antibodies are purified by peptide affinity chromatography. Background Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action (1,2). There are five DUB subfamilies including the USP, UCH, OTU, MJD and JAMM enzymes. USP28 is an important member of the USP subfamily (3). USP28 can bind to and deubiquinate several target proteins in DNA-damage pathway, resulting in their stability, including p53BP1 and Chk2 (4). USP28 also plays an important role in Myc related signaling by binding through FBW7α to Myc . It catalyzes the deubiquitinylation of Myc, thereby promoting its stabilization and contributing to tumor-cell growth (5). Alternate Names Deubiquitinating enzyme 28; KIAA1515; Ubiquitin carboxyl-terminal hydrolase 28; ubiquitin carboxyl-terminal hydrolase 28 variant 1; ubiquitin specific peptidase 28; ubiquitin specific protease 28; Ubiquitin thioesterase 28; ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; UBP28; USP28 Specification REACTIVITY: H M SENSITIVITY: Transfected Only MW (kDa): 135 SOURCE: Rabbit