CST, 42191SF, Stathmin-2 (E3Y2N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying STMN2. Validated for ELISA+. Highly specific and rigorously validated in-house, Stathmin-2 (E3Y2N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #42191) is ready to ship. Product Usage Information This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Stathmin-2 (E3Y2N) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of Stathmin-2. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a fragment of human Stathmin-2 protein. Background Stathmin is a family of microtubule-destabilizing phosphoproteins that play an important role in regulation of the microtubule cytoskeleton (1). The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules, regulating cellular processes, such as cell cycle and neuronal axon development (2). Stathmin family members include stathmin, stathmin-2, stathmin-3, and stathmin-4. Interestingly, stathmin-2 protein levels correlate with decreased TDP43 expression, an mRNA-binding protein that mislocalizes and aggregates in patients with the motor neuron disease, amyotrophic lateral sclerosis (ALS) (3). Interestingly, TDP43 directly regulates stathmin-2 expression by regulating RNA splicing, suggesting that altered stathmin-2 expression might serve as a biomarker for disease progression or a therapeutic target. Alternate Names neuron-specific growth-associated protein; neuronal growth-associated protein (silencer element); Protein SCG10; SCG10; SCGN10; SGC10; stathmin 2; Stathmin-2; stathmin-like 2; STMN2; superior cervical ganglia, neural specific 10; Superior cervical ganglion-10 protein Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG