CST, 42201S, HMGCS1 (D1Q9D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HMGCS1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HMGCS1 (D1Q9D) Rabbit Monoclonal Antibody (CST #42201) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HMGCS1 (D1Q9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMGCS1 protein. This antibody does not cross-react with HMGCS2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu483 of human HMGCS1 protein. Background Cytoplasmic 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1) catalyzes the first committed step of mevalonate pathway essential for cholesterol biosynthesis (1). transcription is regulated by sterol levels (2). Studies showed that miR-223 reduces cholesterol biosynthesis by inhibiting HMGCS1 and methylsterol monooxygenase 1 (3). In addition, activation of the EGFR family member ERBB4 induces the expression of SREBP-regulated genes (including ) involved in cholesterol biosynthesis, suggesting a role of this enzyme in the metabolic re-programming in ERBB4-driven cancers (4). Alternate Names 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 1; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); HMCS1; HMG-CoA synthase; HMGCS; HMGCS1; Hydroxymethylglutaryl-CoA synthase, cytoplasmic; MGC90332 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 57 Source/Isotype: Rabbit IgG