CST, 42235SF, 4E-BP1 (53H11) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying 4E-BP1. Validated for WB,IHC,IF,F,ELISA+. Highly specific and rigorously validated in-house, 4E-BP1 (53H11) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #42235) is ready to ship. Product Usage Information This product is the carrier free version of product #9644. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9644 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity 4E-BP1 (53H11) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total 4E-BP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification 4E-BP1 (53H11) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Alternate Names 4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 15-20 Source/Isotype: Rabbit IgG