CST, 4224S, Tie2 (AB33) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Tie2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Tie2 (AB33) Mouse Monoclonal Antibody (CST #4224) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Tie2 (AB33) Mouse Monoclonal Antibody detects endogenous levels of Tie2 in various endothelial cell lines. This antibody does not cross-react with related proteins. Species Reactivity: Human, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant Tie2 protein fragments corresponding to the extracellular domain of human Tie2. Background Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8). Alternate Names Angiopoietin-1 receptor; CD202B; Endothelial tyrosine kinase; GLC3E; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK; TEK receptor tyrosine kinase; TEK tyrosine kinase, endothelial; TIE-2; TIE2; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; VMCM; VMCM1 Specification REACTIVITY: H B SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Mouse IgG1