CST, 42264T, Caspase-4 (F4T9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CASP4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-4 (F4T9L) Rabbit Monoclonal Antibody (CST #42264) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Caspase-4 (F4T9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total caspase-4 protein. This antibody detects processing intermediate forms of caspase-4 at 40 kDa and 32 kDa, as well as caspase-4 cleavage products at 25 kDa and 22 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-4 protein. Background Caspase-4 (TX/ICH-2/ICE II) is a member of the caspase family of proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Expression of caspase-4 has been observed in most tissues except brain, with highest levels in placenta, lung, spleen, and peripheral blood lymphocytes (PBL). Caspase-4 was originally found to contribute to Fas-mediated apoptosis (4). Several caspases (including caspase-4, caspase-5, and mouse caspase-11 and -12) are most closely related to caspase-1 and are capable of inducing apoptosis when overexpressed but are better characterized in the proteolytic activation of inflammatory cytokines (5). Caspase-4 associates with TRAF6 and is involved in the LPS inducible production of inflammatory cytokines IL-8 and MIP1 in THP-1 cells (6). While caspase-4 and mouse caspase-12 localize to the endoplasmic reticulum (ER) and may be activated by drugs that induce ER stress (7), at least one study suggests that caspase-4 and caspase-12 are not essential for ER stress-induced apoptosis (8). Alternate Names apoptotic cysteine protease Mih1/TX; CASP-4; CASP4; caspase 4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase-4; Caspase-4 subunit 1; Caspase-4 subunit 2; Caspase4; ICE and Ced-3 homolog 2; ICE(rel)-II; ICE(rel)II; ICEREL-II; ICH-2; ICH2; Mih1; Mih1/TX; Protease ICH-2; Protease TX; TX Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 22-25, 32-48 Source/Isotype: Rabbit IgG