CST, 42287T, HSP27 (E5U9P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HSP27 mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HSP27 (E5U9P) Rabbit Monoclonal Antibody (CST #42287) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HSP27 (E5U9P) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSP27 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse HSP27 protein. Background Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). Alternate Names 27kDa; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kDa protein 1; heat shock protein 1; Heat shock protein beta-1; heat shock protein, 25 kDa; Hsp; HSP 25; HSP 27; Hsp25; Hsp27; HspB1; OTTMUSP00000028918; p25; truncated hsp25 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG