CST, 42301S, BARX1 (F2K3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BARX1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, BARX1 (F2K3K) Rabbit Monoclonal Antibody (CST #42301) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BARX1 (F2K3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total BARX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly245 of human BARX1 protein. Background BARX homeobox 1 (BARX1) is a transcription factor (TF) that was first isolated from mouse embryos and identified to function as a repressive TF by directly binding to the NCAM promoter and inhibiting its transcription (1). BARX1 also plays a key role in cellular differentiation and cytoskeletal remodeling in various tissues such as stomach, intestine, and spleen (2-4). Several studies have also established a key role for this TF in the proliferation and metastasis of many tumors. Namely, BARX1 was shown to promote the proliferation of oesophageal adenocarcinoma and renal cell carcinoma (5,6) and was identified as one of the top ten oncogenic TFs that are critical in non-small cell lung cancer (7). Moreover, silencing of BARX1 in endometrial carcinoma cells was shown to suppress ERK/MEK signaling pathway activity and reduce cell viability (8). Alternate Names BarH-like homeobox 1; BARX homeobox 1; BARX1; Homeobox protein BarH-like 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG