CST, 4231S, nNOS (C7D7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying nNOS. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, nNOS (C7D7) Rabbit Monoclonal Antibody (CST #4231) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Frozen): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67860 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity nNOS (C7D7) Rabbit Monoclonal Antibody detects endogenous levels of total nNOS protein. This antibody lacks sensitivity in fixed frozen mouse skeletal muscle by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser280 of human nNOS. Background Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3). Alternate Names bNOS; Constitutive NOS; IHPS1; N-NOS; NC-NOS; Neuronal NOS; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS; NOS type I; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG