CST, 42346S, CCR4 (F9A9K) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying CCR4. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CCR4 (F9A9K) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #42346) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CCR4 (F9A9K) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCR4 protein. Background C-C chemokine receptor type 4 (CCR4) is a chemokine receptor that is primarily expressed in T cells, specifically in Th2, Th17, and Treg subsets (1). Like other chemokine receptors, CCR4 is a G protein-coupled receptor (GPCR) that contains seven transmembrane domains (2). CCR4 selectively binds with the chemokine ligands CCL17, also known as thymus and activation-related chemokine (TARC), and CCL22, also known as macrophage-derived chemokine (MDC). CCL17/TARC functions as a chemoattractant for CCR4-expressing T cells and is associated with type 2 immune responses (3,4). CCL22/MDC is expressed by macrophages and dendritic cells and also functions as a chemoattractant with dominant binding to CCR4 compared with CCL17/TARC (5-7). Elevated CCR4 expression has been implicated in various allergic diseases, such as asthma and dermatitis (8,9). It has also been implicated in several hematological malignancies, including acute myeloid leukemia (AML), acute T cell leukemia/lymphoma (ATLL), and cutaneous T cell lymphoma (CTLL) (1,10). The humanized anti-CCR4 monoclonal antibody mogamulizumab has been approved for the treatment of patients with relapsed ATLL (11). Alternate Names C-C chemokine receptor type 4; C-C CKR-4; C-C motif chemokine receptor 4; CC-CKR-4; CCR-4; CCR4; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4; CMKBR4; HGCN:14099; K5-5; MGC88293 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG