CST, 4234S, nNOS Antibody

Polyclonal Antibody for studying nNOS. Validated for Western Blotting. Highly specific and rigorously validated in-house, nNOS Antibody (CST #4234) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity nNOS Antibody detects endogenous levels of total nNOS protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human nNOS. Antibodies are purified by protein A and peptide affinity chromatography. Background Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3). nNOS binds selectively to the second PDZ domain of PSD-95, and the enzymatic activity of nNOS is either downregulated by phosphorylation at Ser847 by CaM-KII or stimulated by calcium influxes governed by the NMDA receptor channels (4-5). Alternate Names bNOS; Constitutive NOS; IHPS1; N-NOS; NC-NOS; Neuronal NOS; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS; NOS type I; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit