CST, 42353SF, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Stat4 (Tyr693) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #42353) is ready to ship. Product Usage Information This product is the carrier free version of product #4134. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4134 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr693 of human Stat4 protein. Background The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9). Stat4 is activated in response to IL-2 in natural killer (NK) cells, but not in T cells (10). Alternate Names Signal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11; STAT4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 81 Source/Isotype: Rabbit IgG