CST, 42406S, Tom20 (D8T4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TOM20. Validated for WB,IP,IHC,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Tom20 (D8T4N) Rabbit Monoclonal Antibody (CST #42406) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 66401 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Tom20 (D8T4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tom20 protein. This antibody does not have detectable signal by indirect fluorescent detection in frozen mouse tissue. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Tom20 protein. Background Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors TOM20, TOM22, TOM70, and the channel-forming protein TOM40 (1). TOM20 is localized in the outer mitochondrial membrane and initially recognizes precursors with a presequence to facilitate protein import across the outer mitochondrial membrane (2). In a sequential process, recognition of the presequence by TOM20 is followed by tethering of the presequence to the TOM40 protein complex for efficient protein import (3). Alternate Names KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM20; TOMM20; translocase of outer mitochondrial membrane 20; translocase of outer mitochondrial membrane 20 homolog (yeast); translocase of outer mitochondrial membrane 20 homolog type II Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 16 Source/Isotype: Rabbit IgG