CST, 42427T, Helios (D8W4X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Helios. Validated for WB,WB,IP,F,ChIP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, Helios (D8W4X) Rabbit Monoclonal Antibody (CST #42427) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity Helios (D8W4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total Helios protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly73 of human Helios protein. Background Helios (Ikaros family zinc finger 2, IKZF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4). Alternate Names ANF1A2; HELIOS; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; ZNFN1A2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 58, 63-70, 85 Source/Isotype: Rabbit IgG