CST, 42440T, FoxP4 (F5S4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FOXP4. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxP4 (F5S4L) Rabbit Monoclonal Antibody (CST #42440) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FoxP4 (F5S4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxP4 protein. This antibody does not cross-react with FoxP1, FoxP2, or FoxP3 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu230 of human FoxP4 protein. Background Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5). FoxP4 regulates development in heart and lung tissues, and its deletion results in embryonic lethality (5,6). FoxP4 is not required for T cell development, but does play a role in effector T cell cytokine responses in chronic infection (7). Alternate Names FKHLA; FLJ40908; FLJ44184; fork head-related protein like A; Fork head-related protein-like A; forkhead box P4; Forkhead box protein P4; FOXP4; hFKHLA; winged-helix repressor FOXP4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG