CST, 42444S, Phospho-TWIST1 (Ser68) (E8I6K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TWIST1 (Ser68) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-TWIST1 (Ser68) (E8I6K) Rabbit Monoclonal Antibody (CST #42444) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-TWIST1 (Ser68) (E8I6K) Rabbit Monoclonal Antibody recognizes endogenous levels of TWIST1 protein only when phosphorylated at Ser68. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser68 of human phospho-TWIST1 protein. Background TWIST1 is a basic helix-loop-helix (BHLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the BHLH domain of the gene have been associated with Saethre-Chotzen syndrome (SCS), an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in epithelial-mesenchymal transition (EMT) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to taxol and microtubule-regulating drugs in tumors (8). Serine 68 was identified as a major phosphorylation site of TWIST1 and shown to be regulated by mitogen-activated protein kinases (MAPKs) to ultimately stabilize the protein and promote invasiveness in breast cancer cells (9). Alternate Names ACS3; B-HLH DNA binding protein; BHLHA38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; SCS; SWCOS; TWIST; twist basic helix-loop-helix transcription factor 1; twist family bHLH transcription factor 1; twist homolog 1; twist homolog 1 (Drosophila); TWIST homolog of drosophila; Twist-related protein 1; TWIST1; TWST1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG