CST, 4252S, PI3 Kinase p110 gamma Antibody

Polyclonal Antibody for studying PI3K p110 gamma. Validated for Western Blotting. Highly specific and rigorously validated in-house, PI3 Kinase p110 gamma Antibody (CST #4252) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PI3 Kinase p110 gamma Antibody detects endogenous levels of total PI3 kinase p110γ protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human p110gamma. Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP ). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Alternate Names 1-phosphatidylinositol 3-kinase; p110-gamma; p110gamma; p120-PI3K; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidylinositol 3-kinase, catalytic, gamma polypeptide; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; Phosphoinositide-3-kinase catalytic gamma polypeptide; phosphoinositide-3-kinase gamma catalytic subunit; phosphoinositide-3-kinase, catalytic, gamma polypeptide; PI3-kinase subunit gamma; PI3CG; PI3K; PI3K-gamma; PI3Kgamma; PIK3; PIK3CG; PK3CG; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; Serine/threonine protein kinase PIK3CG Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit