CST, 42533S, DR4 (D9S1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Trail-R1/DR4. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, DR4 (D9S1R) Rabbit Monoclonal Antibody (CST #42533) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity DR4 (D9S1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DR4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminal, cytoplasmic domain of human DR4 protein. Background The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR4 (TRAIL-RI, TNFRSF10A) and DR5 (TRAIL-R2, TNFRSF10B) are receptors for the cytokine TRAIL. Both receptors contain death domains that recruit DISC complexes triggering caspase activation and apoptosis (3-6). The ability of TRAIL to selectively kill malignant cells has led to clinical studies involving TRAIL and receptor agonists (7). Alternate Names APO2; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4; MGC9365; TNF receptor superfamily member 10a; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TR10A; TRAIL receptor 1; TRAIL-R1; TRAILR-1; TRAILR1; Tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-55 Source/Isotype: Rabbit IgG