CST, 4262S, Rab10 Antibody

Polyclonal Antibody for studying Rab10. Validated for Western Blotting. Highly specific and rigorously validated in-house, Rab10 Antibody (CST #4262) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rab10 Antibody detects endogenous levels of total Rab10 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp114 of human Rab10 protein. Antibodies are purified by peptide affinity chromatography. Background Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc) and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8). Alternate Names GTP-binding protein RAB10; RAB10; RAB10, member RAS oncogene family; ras-related GTP-binding protein; Ras-related protein Rab-10 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit