CST, 4264S, FXR1 (L662) Antibody

Polyclonal Antibody for studying FXR1. Validated for Western Blotting. Highly specific and rigorously validated in-house, FXR1 (L662) Antibody (CST #4264) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FXR1 (L662) Antibody detects endogenous levels of total FXR1 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu662 of mouse FXR1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation (1). X-linked FMRP (FMR-1) and its two autosomal homologs, FXR1 and FXR2, are polyribosome-associated RNA-binding proteins that are involved in the pathogenesis of fragile X syndrome (1-3). Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). The homolog of FMRP (dFMRP) associates with Argonaute 2 (Ago2) and Dicer and can coimmunoprecipitate with miRNA and siRNA (5). These results suggest that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). Alternate Names FMR1 autosomal homolog 1; Fragile X mental retardation syndrome-related protein 1; fragile X mental retardation, autosomal homolog 1; FXR1; FXR1P; hFXR1p; MYOPMIL; MYORIBF; RNA-binding protein FXR1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 78-80, 82-84 SOURCE: Rabbit