CST, 4265T, STOP (175) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MAP6 mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, STOP (175) Mouse Monoclonal Antibody (CST #4265) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73152 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity STOP (175) Mouse Monoclonal Antibody detects endogenous levels of total N-STOP and O-STOP proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004). Background Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4). Alternate Names 2810411E12Rik; F-S; MAP-6; Map6; Microtubule-associated protein 6; Mta; Mtap6; Stable tubule-only polypeptide; STOP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Mouse IgG1