CST, 4266S, SOD1 (71G8) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SOD1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SOD1 (71G8) Mouse Monoclonal Antibody (CST #4266) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SOD1 (71G8) Mouse Monoclonal Antibody detects endogenous levels of SOD1 protein. This antibody does not cross-react with other related proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein. Background SOD1, Cu/Zn superoxide dismutase, is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen (1). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space. The SOD1 gene locus is on chromosome 21 in a region affected in Down Syndrome (2). In addition, over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons (3-5). Despite the fact that SOD1 helps to eliminate toxic reactive species, its mutations in ALS have been described as gain-of-function (5). The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear. Mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight species that aggregate and end up in proteinaceous inclusions (5). Alternate Names ALS; ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; homodimer; hSod1; indophenoloxidase A; IPOA; SOD; SOD, soluble; SOD1; SODC; STAHP; superoxide dismutase; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; superoxide dismutase, cystolic Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Mouse IgG1