CST, 4270S, Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) Antibody

Polyclonal Antibody for studying Catenin-beta (Ser33/Ser37/Thr41) non-phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) Antibody (CST #4270) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) Antibody recognizes endogenous β-Catenin when residues Ser33, Ser37, and Thr41 are not phosphorylated. This antibody does not detect β-Catenin if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-Catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography. Background β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Alternate Names armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 92 SOURCE: Rabbit