CST, 42713S, THEM2 Antibody

Polyclonal Antibody for studying THEM2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, THEM2 Antibody (CST #42713) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity THEM2 Antibody recognizes endogenous levels of total THEM2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEM2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background THEM2 is a homotetrameric fatty acyl-CoA thioesterase (1). THEM2 and PC-TP (phosphatidylcholine transfer protein), both enriched in liver, interact to form a complex (1). Cell membrane-bound phosphatidylcholines bind to PC-TP in the complex (1). The complex in turn inhibits IRS-2 and mTORC1, which leads to the suppression of insulin signaling (1). THEM2 has also been shown to regulate adaptive thermogenesis in mice (2). Alternate Names ACO13; ACOT13; Acyl-CoA thioesterase 13; Acyl-coenzyme A thioesterase 13; Acyl-coenzyme A thioesterase 13, N-terminally processed; HT012; hypothalamus protein HT012; MGC4961; PNAS-27; THEM2; Thioesterase superfamily member 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 15 SOURCE: Rabbit