CST, 4272S, Cytochrome c Antibody

Polyclonal Antibody for studying cytochrome C. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome c Antibody (CST #4272) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Cytochrome c Antibody detects endogenous levels of cytochrome c protein. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c. Antibodies are purified by protein A and peptide affinity chromatography.    Background Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). Alternate Names CYC; CYCS; Cytochrome c; cytochrome c, somatic; HCS; THC4 Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous MW (kDa): 14 SOURCE: Rabbit