CST, 42733T, TFE3 (F3Z2Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TFE3. Validated for WB,WB,IP,IHC,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, TFE3 (F3Z2Q) Rabbit Monoclonal Antibody (CST #42733) is ready to ship. Product Usage Information For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:125 - 1:500 Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Chromatin IP Specificity / Sensitivity TFE3 (F3Z2Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total TFE3 protein. In addition to the canonical TFE3 protein P19532, this antibody also detects the homologous protein B4DIA5. This antibody does not cross-react with TFEB or MITF proteins. Non-specific salivary gland and pancreatic islet associated staining was observed by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu513 of human TFE3 protein. Background Transcription factor E3 (TFE3) is a member of a family of basic helix-loop-helix leucine zipper transcription factors that include MITF, TFEB, TFE3, and TFEC. Members of this family form heterodimers with each other, bind the same DNA sequences, and undergo the same types of post-translational modifications, including sumoylation (1). Research studies indicate that TFE3 and other family members play roles in development, organelle biogenesis, nutrient sensing, autophagy, and energy metabolism (2,3). Additional studies report that TFE3 controls the gate for pluripotent cells to exit the state of pluripotency prior to differentiation (4). Translocations involving the gene region have been identified in a number of tumors, including sporadic renal cell tumors. Several specific translocations that result in kidney cancer and involve the gene have been described and characterized in detail (5). Alternate Names BHLHE33; class E basic helix-loop-helix protein 33; DKFZp761J1810; Putative uncharacterized protein DKFZp761J1810; RCCP2; RCCX1; TFE3; TFEA; transcription factor binding to IGHM enhancer 3; transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; transcription factor for immunoglobulin heavy-chain enhancer 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 72-89 Source/Isotype: Rabbit IgG