CST, 42738SF, CEA/CD66e (CB30) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CEACAM5. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, CEA/CD66e (CB30) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #42738) is ready to ship. Product Usage Information This product is the carrier free version of product #2383. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2383 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CEA/CD66e (CB30) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of CEA protein. Species Reactivity: Human Source / Purification Monoclonal antibody (Isotype: IgG1) is produced by immunizing mice with human CEA. Background Carcinoembryonic antigen (CEA), also known as CD66e or CEACAM5, is a 180-200 kDa cell surface glycoprotein whose expression is elevated in intestinal carcinomas and other tumors. CEA mediates cell adhesion, though little more is known about its biological activity. Expression of CEA is correlated with tumerogenicity (1), and it has been shown to play a role in cell migration, adhesion and invasion in culture cells, as well as in metastasis in vivo (2). Alternate Names Carcinoembryonic antigen; carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; CEA cell adhesion molecule 5; CEACAM5; CEAM5; DKFZp781M2392; Meconium antigen 100 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 180-200 Source/Isotype: Mouse IgG1