CST, 42746S, Layilin (E9U6M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAYN. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Layilin (E9U6M) Rabbit Monoclonal Antibody (CST #42746) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Layilin (E9U6M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Layilin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Layilin protein. Background Layilin (LAYN) is a single-pass type I integral membrane protein widely expressed by different cell types and tissues (1). LAYN is a hyaluronan receptor that directly interacts with several cytoskeletal-membrane linker molecules, including talin, merlin, and radixin (1-3). In both tumor and immune cell types, LAYN plays an important role in cell adhesion and motility, promoting cell spreading, migration, and infiltration (1,4-7). LAYN is involved in the tumor necrosis factor-α induced epithelial-mesenchymal transition of renal tubular epithelial cells (8), and also plays a critical role in HA35-induced intestinal epithelial tight junctions in inflammatory bowel disease (9). Lineage-specific deletion of LAYN on murine CD8  T cells reduced their accumulation in tumors and increased tumor growth (10). Multiple isoforms have been identified. Alternate Names FLJ30977; FLJ31092; Layilin; LAYN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43, 55 Source/Isotype: Rabbit IgG