CST, 4275S, Granzyme B Antibody

Polyclonal Antibody for studying Granzyme B. Validated for Western Blotting. Highly specific and rigorously validated in-house, Granzyme B Antibody (CST #4275) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Granzyme B Antibody detects endogenous levels of total Granzyme B protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a central region of Granzyme B. Antibodies were purified by protein A and peptide affinity chromatography. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Alternate Names C11; Cathepsin G-like 1; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA-1; CTLA1; CTSGL1; cytotoxic serine protease B; Cytotoxic T-lymphocyte proteinase 2; fragmentin 2; Fragmentin-2; GRAB; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GRB; GZMB; HLP; Human lymphocyte protein; Lymphocyte protease; SECT; T-cell serine protease 1-3E Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 30-37 SOURCE: Rabbit