CST, 42786SF, GAP43 (D9C8) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GAP43/Neuromodulin. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, GAP43 (D9C8) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #42786) is ready to ship. Product Usage Information This product is the carrier free version of product #8945. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8945 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GAP43 (D9C8) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GAP43 protein. Low levels of non-specific nuclear labeling may be observed by immunocytochemistry (ICC). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys30 of human GAP43 protein. Background GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7). Alternate Names Axonal membrane protein GAP-43; B-50; calmodulin-binding protein P-57; GAP-43; GAP43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; NEUM; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; pp46; protein F1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38, 43 Source/Isotype: Rabbit IgG