CST, 42839S, Axl (F3K7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Axl mouse. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Axl (F3K7B) Rabbit Monoclonal Antibody (CST #42839) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Axl (F3K7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Axl protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Axl protein. Background Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4). Alternate Names Adhesion-related kinase; AI323647; Ark; Axl; AXL receptor tyrosine kinase; OTTMUSP00000025228; Tyr; Tyro7; Tyrosine-protein kinase receptor UFO; Ufo; ufo oncogene homolog Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG