CST, 4294S, PHLDA3 Antibody

Polyclonal Antibody for studying PHLDA3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PHLDA3 Antibody (CST #4294) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PHLDA3 Antibody detects endogenous level of total PHLDA3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PHLDA3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Pleckstrin homology-like domain family A member 3 (PHLDA3) is one of three relatively small, similar proteins that share a common pleckstrin homology (PH) domain. Expression of PHLDA3 and the related PHLDA1 occurs in several fetal and adult tissues, in contrast to the more restricted expression of PHLDA2 seen in mouse tissues (1). PHLDA3 is one of several proteins induced following treatment of tumor cells with cisplatin, an anti-cancer drug that cross-links DNA and promotes apoptosis through activation of the tumor suppressor p53 (2). Additional evidence that PHLDA3 is involved in promoting apoptosis through p53 came from a study examining the opposing effects of p53 and Akt in tumor development. Tumor suppressor p53 binds the PHLDA3 promoter to induce transcription. Induced overexpression of PHLDA3 increases apoptosis while deletion of PHLDA3 results in increased Akt activity and a reduction in p53-mediated apoptosis. PHLDA3 appears to compete with the PH domain of Akt, preventing Akt activation and promotion of Akt-induced cell survival pathways (3). Alternate Names PHLA3; PHLDA3; pleckstrin homology like domain family A member 3; Pleckstrin homology-like domain family A member 3; pleckstrin homology-like domain, family A, member 2; pleckstrin homology-like domain, family A, member 3; TDAG51/Ipl homolog 1; TIH1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 15 SOURCE: Rabbit