CST, 4300S, PDAP1 Antibody

Polyclonal Antibody for studying PDAP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PDAP1 Antibody (CST #4300) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PDAP1 Antibody detects endogenous levels of total PDAP1 protein. Nonspecific bands are seen above 60 KDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the center of human PDAP1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Platelet derived growth factor (PDGF) proteins function as dimeric isoforms (i.e., PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind receptor tyrosine kinases and activate cytoplasmic SH2 domain-containing proteins to control multiple signaling pathways that regulate angiogenesis, cell growth, actin reorganization, migration and differentiation (1). PDGFA-associated protein 1 (PDAP1) was originally identified as a novel, PDGF-associated protein found in a rat retinal tumor cell line (2). While copurified with PDGFA, PDAP1 interacts with PDGFB at a slightly higher affinity than with PDGFA (2). Although the exact function of PDAP1 is unclear, it has been shown to both increase PDGFA-induced incorporation of [ H]thymidine in Swiss 3T3 cells and decrease PDGFB growth factor activity (2). Ubiquitously expressed PDAP1 is highly conserved among species (2,3) and is phosphorylated by several kinases, including PKC, PKA, CKI and CKII. Among this group, CKII seems to be the major kinase that phosphorylates PDAP1 in intact cells (3). Alternate Names 28 kDa heat- and acid-stable phosphoprotein; HAP28; HASPP28; PAP; PAP1; PDAP1; PDGF associated protein; PDGF-associated protein; PDGFA associated protein 1; PDGFA-associated protein 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit