CST, 43035T, ZHX2 (E5H5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZHX2. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ZHX2 (E5H5G) Rabbit Monoclonal Antibody (CST #43035) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity ZHX2 (E5H5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZHX2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ZHX2 protein. Background Zinc fingers and homeoboxes protein 2 (ZHX2) is a transcriptional repressor and tumor suppressor that belongs to the zinc fingers and homeoboxes (ZHX) protein family, which also contains ZHX1 and ZHX3 (1). ZHX proteins are expressed only in vertebrates and contain two C H zinc finger motifs as well as five homeodomains, which are involved in DNA-protein and protein-protein interactions (2). ZHX2 has been shown to form homodimers as well as heterodimers with ZHX1, and also interacts with nuclear factor Y subunit alpha (NF-YA) (2). Through these interactions, ZHX2 functions as a transcriptional repressor by reducing the expression levels of a number of genes including cyclin A, cyclin E, and alpha-fetoprotein, among others (2-4). ZHX2 functions as a tumor suppressor in some tissues, as loss of ZHX2 leads to hepatocellular carcinoma, lymphoma, and myeloma (4). In clear cell renal carcinoma (ccRCC), ZHX2 appears to function as an oncogene, as loss of von Hippel-Lindau protein (VHL) leads to increased expression of ZHX2 and increased cellular proliferation (5). ZHX2 also contributes to atherosclerosis by promoting macrophage survival and pro-inflammatory function, leading to growth of atherosclerotic lesions (6). Additionally, ZHX2 functions to promote the maintenance of neural progenitor cells in the brain through repression of ephrin-B (7). Alternate Names AFP regulator 1; AFR1; Alpha-fetoprotein regulator 1; KIAA0854; RAF; Regulator of AFP; transcription factor ZHX2; ZHX2; Zinc finger and homeodomain protein 2; zinc fingers and homeoboxes 2; Zinc fingers and homeoboxes protein 2; zinc-fingers and homeoboxes 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG