CST, 43043S, TRIB3 (E7G5P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tribbles3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIB3 (E7G5P) Rabbit Monoclonal Antibody (CST #43043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRIB3 (E7G5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIB3 protein. This antibody does not cross-react with other TRIBBLES family proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu331 of human TRIB3 protein. Background TRIBBLES proteins belong to a small family of serine-threonine kinase-like proteins characterized by the presence of a variant protein kinase motif (lacking a canonical ATP binding site), a MEK1 binding site, and a C-terminal COP1 site that binds ubiquitin ligase. The gene was first identified and characterized in genetic screens for genes that regulate cell division, gastrulation, and oogenesis (1-3). Research studies in suggested that TRIBBLES functions to coordinate cell division by regulating turnover of the cell cycle protein String/cdc25. In contrast to the genome, which contains a single gene, the genomes of mice and humans encode three known TRIBBLES proteins (TRIB1-3), which exhibit both distinct and overlapping patterns of expression and functions (4). For example, TRIB1 and TRIB2, but not TRIB3, were reported to promote degradation of the basic region-leucine zipper transcription factor C/EBPα, a function that appears to be conserved from flies to humans (5,6). TRIB2 is overexpressed in a subset of human AML patient samples, downregulated in leukemic cells undergoing proliferation arrest (7), and positively regulated by the NOTCH signaling pathway in T cells (8), while retroviral-mediated overexpression of TRIB2 in mice was shown to induce transplantable leukemia (7). These findings collectively suggest that TRIB2 functions as an oncogene in the mammalian hematopoietic system (9). Enhanced TRIB3 expression has been associated with cancer development, cancer radiotherapy resistance, as well as liver fibrosis (10,11). The protein mainly functions by interacting with a wide variety of key regulators, such as EGFR, Akt, β-catenin, TAZ, and SQSTM1, to promote their functions in these disease development processes (11-14). Alternate Names C20orf97; neuronal cell death inducible putative kinase; Neuronal cell death-inducible putative kinase; NIPK; p65-interacting inhibitor of NF-kappa-B; p65-interacting inhibitor of NF-kappaB; SINK; SKIP3; TRB-3; TRB3; TRIB3; Tribbles homolog 3; tribbles homolog 3 (Drosophila); tribbles pseudokinase 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG