CST, 43069S, Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ITSN2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody (CST #43069) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Intersectin-2 (E5J9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total intersectin-2 protein. This antibody reacts with intersectin-2L and intersectin-2S protein isoforms but does not cross-react with the corresponding isoforms of intersectin-1 protein. This antibody cross-reacts with proteins of unknown identity between 80 kDa and 120 kDa in some rat cell extracts. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys335 of human intersectin-2 protein. Background Intersectin-2 is a ubiquitously expressed, modular adaptor protein that participates in clathrin-mediated endocytosis through interaction with multiple proteins involved in trafficking of proteins through the endocytic pathway. Like its paralog, intersectin-1, there are two major isoforms of mammalian intersectin-2. Intersectin-2L and intersectin-2S are generated through alternative splicing of mRNA transcripts. The structure of both isoforms is characterized by two N-terminal EH domains, a central coiled-coil domain, followed by five tandem SH3 domains. The longer isoform, intersection-2L, contains an extended C-terminus with a Rho-GEF domain that controls the activity of the Cdc42 GTPase, a PH domain, and a calcium binding C2 domain (1-3). Research studies have shown that intersectin-2 plays a role in T cell receptor (TCR) signaling by promoting endocytosis of the TCR following its activation (4). Alternate Names intersectin 2; Intersectin-2; ITSN2; KIAA1256; PRO2015; SH3 domain protein 1B; SH3 domain-containing protein 1B; SH3D1B; SH3P18; SH3P18-like WASP associated protein; SH3P18-like WASP-associated protein; SWA; SWAP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200, 140 Source/Isotype: Rabbit IgG