CST, 43079S, BOB-1/OBF-1 (E5K1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POU2AF1. Validated for WB,IP,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, BOB-1/OBF-1 (E5K1D) Rabbit Monoclonal Antibody (CST #43079) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 65452 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity BOB-1/OBF-1 (E5K1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total BOB-1/OBF-1 protein. Non-specific staining was observed in pancreatic islets by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro209 of human BOB-1/OBF-1 protein. Background B cell Oct binding factor-1 (BOB-1/OBF-1) is a B cell restricted transcriptional coactivator. BOB-1 facilitates transactivation of immunoglobulins and other B cell specific genes through the binding and activation of the transcription factors Oct-1 and Oct-2 (1-4). Research studies have demonstrated that BOB-1 expression is required for antigen-dependent B cell maturation (5-7). In pathological conditions such as classical Hodgkin disease, loss of BOB-1 expression is thought, in part, to contribute to the defect in immunoglobulin gene expression by Hodgkin and Reed Sternberg cells (8,9). In the context of multiple myeloma, overexpression of BOB-1 has been shown to contribute to malignant plasma cell growth, in part, through enhanced transactivation of (10). Alternate Names B-cell-specific coactivator OBF-1; BOB-1; BOB1; OBF-1; OBF1; OCA-B; OCAB; OCT-binding factor 1; POU class 2 associating factor 1; POU class 2 homeobox associating factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1; POU2AF1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG