CST, 4307T, Thymidine Phosphorylase/ECGF1 (D69B12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying thymidine phosphorylase. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Thymidine Phosphorylase/ECGF1 (D69B12) Rabbit Monoclonal Antibody (CST #4307) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Thymidine Phosphorylase/ECGF1 (D69B12) Rabbit Monoclonal Antibody detects endogenous levels of total TP/ECGF1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln370 of human thymidine phosphorylase/ECGF1 protein. Background Thymidine phosphorylase (TP) is a platelet-derived endothelial cell growth factor (PD-ECGF) that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate (1). This intracellular enzyme is capable of both promoting angiogenesis and inhibiting apoptosis. Thymidine phosphorylase catalytic activity is required for its angiogenic function (2,3). Increased expression of TP/PD-ECGF is seen in a wide variety of different solid tumors and inflammatory diseases and is often associated with poor prognosis (4,5). Alternatively, TP can activate fluorouracil derivative (DFUR) prodrugs and increase the antitumor activity of the related treatment (1,5). The use of thymidine phosphorylase as a cancer therapeutic target has been studied extensively, with emphasis on either inhibiting TP enzymatic activity or increasing enzyme induction with concomitant DFUR treatment (1,5). Alternate Names ECGF; ECGF1; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PD-ECGF; PDECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine phosphorylase; TP; TYMP; TYPH Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit