CST, 43098S, Myogenin (E9A1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYOG. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Myogenin (E9A1S) Rabbit Monoclonal Antibody (CST #43098) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 29510 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Myogenin (E9A1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total myogenin protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu146 of human myogenin protein. Background Myogenin (MYOG), also called myogenic factor 4 (Myf-4), is a transcriptional regulatory protein that belongs to the myogenic basic helix-loop-helix protein family, which also includes MyoD, Myf-5, and MRF4 (1). It is involved in skeletal muscle development and differentiation and is a marker for myogenesis (1,2). Detection of aberrant myogenin expression is also useful in pathology as an indicator of rhabdomyosarcoma (RMS) (2,3). Alternate Names BHLHC3; Class C basic helix-loop-helix protein 3; Myf-4; MYF4; MYOG; Myogenic factor 4; Myogenic factor-4; Myogenin; myogenin (myogenic factor 4) Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG