CST, 43163SF, Phospho-Ubiquitin (Ser65) (E5T1W) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying UBA52 (Ser65) phosphate/UBC (Ser65) phosphate/UBB (Ser65) phosphate/RPS27A (Ser65) phosphate. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Ubiquitin (Ser65) (E5T1W) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #43163) is ready to ship. Product Usage Information This product is the carrier free version of product #70973. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 70973 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Ubiquitin (Ser65) (E5T1W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of Ubiquitin protein only when phosphorylated at Ser65. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser65 of human Ubiquitin protein. Background Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7). Ubiquitin is phosphorylated at Ser65 by PINK1, leading to activation of the E3 ubiquitin ligase Parkin (8, 9). PINK1 accumulates on depolarized mitochondria, resulting in phosphorylation of ubiquitin and activation of Parkin, which then triggers the mitophagy pathway to clear damaged mitochondria. Loss-of-function mutations in PINK1 and Parkin result in early onset Parkinson's disease (10, 11). Alternate Names 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; CEP80; epididymis luminal protein 112; epididymis secretory protein Li 50; FLJ25987; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; Large ribosomal subunit protein eL40; MGC126879; MGC126881; MGC57125; MGC8385; polyubiquitin B; Polyubiquitin-B; Polyubiquitin-C; ribosomal protein L40; ribosomal protein S27a; RL40; RPL40; RPS27A; RS27A; S27A; Small ribosomal subunit protein eS31; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; UBCEP80; ubiquitin; Ubiquitin; ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein L40; ubiquitin and ribosomal protein S27a; Ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; Ubiquitin-40S ribosomal protein S27a; ubiquitin-52 amino acid fusion protein; Ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG