CST, 4317S, FMRP Antibody

Polyclonal Antibody for studying FMRP. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, FMRP Antibody (CST #4317) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FMRP Antibody detects endogenous levels of total FMRP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr559 of human FMRP. Antibodies are purified by protein A and peptide affinity chromatography. Background Fragile X syndrome, a frequent cause of inherited mental retardation, often results from expansion of the CGG trinucleotide repeat in the gene that encodes the fragile X mental retardation protein (FMRP) (1). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (1-3). Each of these related proteins can associate with one another as well as form homodimers (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). In , dFMRP associates with Argonaute 2 (Ago2) and Dicer and coimmunoprecipitates with miRNA and siRNA. These results suggest that fragile X syndrome is related to abnormal translation caused by a defect in RNAi-related pathways (5). In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). Alternate Names FMR1; FMRP; FMRP translational regulator 1; fragile X mental retardation 1; Fragile X mental retardation 1 protein; Fragile X mental retardation protein 1; Fragile X messenger ribonucleoprotein 1; FRAXA; MGC87458; POF; POF1; premature ovarian failure 1; Protein FMR-1; Synaptic functional regulator FMR1; truncated FMRP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 SOURCE: Rabbit