CST, 43188SF, LCP1 (D1C3) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LCP1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, LCP1 (D1C3) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #43188) is ready to ship. Product Usage Information This product is the carrier free version of product #3588. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3588 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LCP1 (D1C3) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LCP1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp567 of human LCP1 protein. Background Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mammals and include L-plastin, T-plastin, and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues, while I-plastin (plastin-1) is expressed specifically in the kidney, colon, and small intestine (1-3). Research studies have shown that L-plastin (plastin-2) or lymphocyte cytosolic protein 1 (LCP1) is mainly expressed in hematopoietic cells and nonhematopoietic tumors, and increased expression correlates with metastatic progression in colon cancer cell lines (4). Investigators have found that overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T cell receptor/CD3 complex in association with the CD2 cell adhesion molecule or the CD28 receptor (6). Phosphorylation at Ser5 enhances the ability of LCP1 to bind to F-actin and increases cell motility (7,8). Alternate Names bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)); CP64; DKFZp781A23186; epididymis secretory protein Li 37; FLJ25423; FLJ26114; FLJ39956; HEL-S-37; L-plastin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P); LC64P; LCP-1; LCP1; LPL; Lymphocyte cytosolic protein 1; lymphocyte cytosolic protein 1 (L-plastin); Lymphocyte cytosolic protein-1 (plasmin); plastin 2; Plastin-2; PLS2; PLSL Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG