CST, 43248T, PD-1 (EH33) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PDCD1. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PD-1 (EH33) Mouse Monoclonal Antibody (CST #43248) is ready to ship. Product Usage Information IHC Leica Bond: 1:100 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and Azide) version of this product see product #www.cellsignal.com/products/primary-antibodies/pd-1-eh33-mouse-mab-ihc-specific-bsa-and-azide-free/60847?_=1653488406131&Ntt=PD-1%20(EH33)%20Mouse%20mAb%20(IHC-Specific)&tahead=true">60847. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity PD-1 (EH33) Mouse Monoclonal Antibody recognizes transfected and endogenous levels of total PD-1 protein by immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PD-1 protein. Background The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10). Alternate Names CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; programmed cell death 1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; SLEB2; systemic lupus erythematosus susceptibility 2 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2a