CST, 43258SF, Cleaved Caspase-7 (Asp198) (D6H1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Casp7 (Asp198, 20kD) cleaved. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Cleaved Caspase-7 (Asp198) (D6H1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #43258) is ready to ship. Product Usage Information This product is the carrier free version of product #8438. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8438 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cleaved Caspase-7 (Asp198) (D6H1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of caspase-7 protein only when cleaved at Asp198. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp198 of human caspase-7 protein. Background Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5). Alternate Names Apoptotic protease Mch-3; CASP-7; CASP7; caspase 7; caspase 7 isoform delta; caspase 7, apoptosis-related cysteine peptidase; caspase 7, apoptosis-related cysteine protease; Caspase-7; Caspase-7 subunit p11; Caspase-7 subunit p20; caspase7; CMH-1; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; Lice2 alpha/beta/gamma; MCH3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG