CST, 43278S, Dynamin (pan) (F2E9N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DYN1/DYN2/DYN3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Dynamin (pan) (F2E9N) Rabbit Monoclonal Antibody (CST #43278) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Dynamin (pan) (F2E9N) Rabbit Monoclonal Antibody recognizes endogenous levels of total dynamin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly495 of human dynamin protein. Background Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. There are three isoforms of dynamin. Dynamin 1 and 3 expression is enriched in the brain, while dynamin 2 expression is ubiquitous. All three isoforms mediate membrane fission during clathrin-mediated endocytosis. Dynamin 2 is also suggested to be involved in non-clathrin-mediated endocytosis and cytoskeletal interactions (2-4). Dynamin 3 is suggested to regulate AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor recycling through its interaction with the postsynaptic density scaffolding proteins Homer and Shank (5-7). In addition to the role they play in neurotransmission, dynamin family members are considered therapeutic targets in cancer. Dysregulation of endocytosis in cancer leads to dysfunction in cell signaling, survival, and proliferation. Dynamin 1 and 2 have been shown to promote tumorigenesis, while dynamin 3 is a suggested tumor suppressor (8-12). Alternate Names CMT2M; CMTDI1; CMTDIB; DI-CMTB; DNM; DNM1; DNM2; DNM3; DYN1; DYN2; DYN3; Dyna III; dynamin 1; dynamin 2; dynamin 3; dynamin family member; dynamin II; Dynamin-1; Dynamin-2; Dynamin-3; Dynamin, testicular; DYNII; EIEE31; KIAA0820; LCCS5; MGC70433; T-dynamin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG