CST, 43339T, FLASH/CASP8AP2 (D3T8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CASP8AP2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FLASH/CASP8AP2 (D3T8Q) Rabbit Monoclonal Antibody (CST #43339) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FLASH/CASP8AP2 (D3T8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total FLASH/CASP8AP2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to residues near the amino terminus of human FLASH/CASP8AP2 protein. Background FLICE associated huge protein (FLASH), also termed Caspase 8 associated protein 2 (CASP8AP2), is a large multifunctional protein that has been shown to be involved in multiple processes, including apoptosis, cell cycle progression, and transcriptional regulation (1-3). It was originally identified as a component of the FAS-FADD-Caspase 8 complex with a role in the activation of caspase-8 during Fas-mediated apoptosis (1). However, studies suggest additional functions for FLASH/CASP8AP2 in the nucleus within Cajal and PML bodies (3-5). One of the functions identified was its ability to regulate histone gene processing and S-phase progression (3,6). FLASH/CASP8AP2 has also been found to regulate the activity of transcription factors, including the glucocorticoid receptor and c-Myb (2,7). Furthermore, FLASH/CASP8AP2 was found to stabilize the expression of ZEB1, a transcriptional repressor of genes associated with epithelial-to-mesenchymal transition (EMT), a process associated with cancer metastasis (8). Aberrant expression as well as genetic mutations in the gene have been described in several cancers and it has been suggested to be a prognostic indicator in acute lymphoblastic leukemia (9-14). Alternate Names C8AP2; CASP8 associated protein 2; CASP8-associated protein 2; CASP8AP2; caspase 8 associated protein 2; CED-4; FLASH; FLASH homolog RIP25; FLICE associated huge; FLICE-associated huge protein; FLJ11208; human FLASH; KIAA1315; RIP25 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 223 Source/Isotype: Rabbit IgG