CST, 43352W2, Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 532 Conjugate)

Monoclonal Antibody for studying PI3K p85 alpha (Tyr467) phosphate/PI3K p55 (Tyr199) phosphate/PI3K p85 beta (Tyr464) phosphate. Highly specific and rigorously validated in-house, Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 532 Conjugate) (CST #43352) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr467 (equivalent to Tyr458 in mouse) of human p85α protein. Background Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP ). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Protein extracts from 3T3-Src cells were profiled by PhosphoScan to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5). Alternate Names 100% homology to SWISS-PROT Q92569; AGM7; DKFZp686P05226; FLJ41892; GRB1; IMD36; MPPH; MPPH1; p55; p55-GAMMA; P55G; p55PIK; p85; p85-ALPHA; p85-BETA; P85A; P85B; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase, regulatory, 1; phosphoinositide-3-kinase regulatory subunit 1; phosphoinositide-3-kinase regulatory subunit 2; phosphoinositide-3-kinase regulatory subunit 3; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit beta; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3-kinase subunit p85-beta; PI3K regulatory subunit alpha; PI3K regulatory subunit beta; PI3K regulatory subunit gamma; PIK3R1; PIK3R2; PIK3R3; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit beta; PtdIns-3-kinase regulatory subunit gamma; PtdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-beta Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG