CST, 43411S, REG4 Antibody

Polyclonal Antibody for studying REG4. Validated for Western Blotting. Highly specific and rigorously validated in-house, REG4 Antibody (CST #43411) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity REG4 Antibody recognizes endogenous levels of total human REG4 protein. REG4 antibody may weakly bind mouse Reg4. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human REG4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Reg4 (regenerating islet-derived protein 4) is a member of a multigene family of Reg proteins (1). Reg proteins are secreted glycoproteins belonging to the calcium (C-type) dependent lectin superfamily, although they can in fact bind polysaccharides, mannan, and heparin in the absence of calcium (2,3). Reg4 is expressed in the gastrointestinal (GI) tract, in normal colon mucosa, and is upregulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease (Crohn's disease and ulcerative colitis) (4,5). It has recently been shown that Reg4 marks a population of deep secretory cells at the bottom of the crypts in the colon. In the upper gastrointestinal tract, Paneth cells support the Lgr5+ positive stem cells that reside at the base of deep crypts by providing signals such as Wnt3, EGF, and Notch ligands for stem cell maintenance. No Paneth cells, however, exist in the colon crypts. It is proposed that the Reg4+ population serves as an analogous support system for the LGR5+ positive stem cell population in the colon (1,6). Alternate Names Gastrointestinal secretory protein; GISP; Reg IV; REG-4; REG-IV; REG-like protein; REG4; regenerating family member 4; regenerating gene type IV; regenerating islet-derived family, member 4; Regenerating islet-derived protein 4; Regenerating islet-derived protein IV; RELP Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 14 SOURCE: Rabbit