CST, 43424S, CPA3 (F9F2X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CPA3. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CPA3 (F9F2X) Rabbit Monoclonal Antibody (CST #43424) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CPA3 (F9F2X) Rabbit Monoclonal Antibody recognizes endogenous levels of total CPA3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser318 of human CPA3 protein. Background Carboxypeptidase A3 (CPA3) is an enzyme that plays a role in remodeling the extracellular matrix, and it is capable of cleaving several targets, including angiotensin I, apolipoprotein B, and endothelin-1 (1). CPA3 expression is largely limited to secretory granules within specific subsets of mast cells, but has also been reported in basophils in the context of allergic conditions (2,3). Although CPA3 protein is expressed only in mast cells that also express tryptase and mast cell chymase (CMA1) (MC ), the gene may be expressed in both MC and mast cells that express tryptase but not CMA1 (MC ) (4). Increased CPA3 expression is associated with several adverse health conditions, including certain subtypes of acute myeloid leukemia, asthma, severe COVID-19, chronic obstructive pulmonary disease, and idiopathic pulmonary fibrosis (1,5-7). Alternate Names Carboxypeptidase A3; carboxypeptidase A3 (mast cell); CBPA3; CPA3; Mast cell carboxypeptidase A; MC-CPA; tissue carboxypeptidase A Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG