CST, 4357T, RagA (D8B5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RagA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RagA (D8B5) Rabbit Monoclonal Antibody (CST #4357) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RagA (D8B5) Rabbit Monoclonal Antibody detects endogenous levels of total RagA protein. It may also detect endogenous levels of total RagB protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn72 of human RagA protein. Background The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). Alternate Names Adenovirus E3 14.7 kDa-interacting protein 1; adenovirus E3-14.7K interacting protein 1; FIP-1; FIP1; Rag A; RagA; Ras related GTP binding A; Ras-related GTP binding A; Ras-related GTP-binding protein A; RRAGA Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG